Digital Microfluidic qPCR Cartridge for SARS-CoV-2 Detection

Micromachines

Yen-Wen Lu¹  Hong-Yu Liao²  Kuan-Lun Ho³  Shih-Kang Fan³  Justin Yu Chang⁴  Pin-Kuan Yeh⁴  Helene Minyi Liu⁵ 

[1] Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, Taipei 10617, Taiwan;Department of Mechanical Engineering, National Taiwan University, Taipei 10617, Taiwan;Department of Mechanical and Nuclear Engineering, Kansas State University, Manhattan, KS 66506, USA;GEMFluidix LLC, Los Angeles, CA 91748, USA;Graduate Institute of Biochemistry and Molecular Biology, National Taiwan University, Taipei 10052, Taiwan;

关键词: SARS-CoV-2;    COVID-19;    droplet qPCR;    digital microfluidics;    electrowetting;   

DOI  :  10.3390/mi13020196

来源: DOAJ

【 摘 要 】

Point-of-care (POC) tests capable of individual health monitoring, transmission reduction, and contact tracing are especially important in a pandemic such as the coronavirus disease 2019 (COVID-19). We develop a disposable POC cartridge that can be mass produced to detect the SARS-CoV-2 N gene through real-time quantitative polymerase chain reaction (qPCR) based on digital microfluidics (DMF). Several critical parameters are studied and improved, including droplet volume consistency, temperature uniformity, and fluorescence intensity linearity on the designed DMF cartridge. The qPCR results showed high accuracy and efficiency for two primer-probe sets of N1 and N2 target regions of the SARS-CoV-2 N gene on the DMF cartridge. Having multiple droplet tracks for qPCR, the presented DMF cartridge can perform multiple tests and controls at once.

【 授权许可】

Unknown