Analysis of the release kinetics of surface-bound proteins via laser-induced fluorescence

Current Directions in Biomedical Engineering

Dangers Marc¹  Pollack Thomas²  Seitz Hermann² 

[1] DST Diagnostische Systeme & Technologien GmbH, Güterbahnhofstraße 16 / 19059 Schwerin, Germany;University of Rostock, Fluid Technology and Microfluidics, Justus-von-Liebig Weg 6, 18059 Rostock, Germany;

关键词: laser-induced fluorescence;    spectroscopy;    release kinetics;    point-of-care testing (poct);   

DOI  :  10.1515/cdbme-2015-0084

来源: DOAJ

【 摘 要 】

The drying and resolving processes of surface-bound proteins were analysed with a stereoscopic microscope combined with laser-induced fluorescence (LIF) with the aim to assist the advancement of a semiautomated point-of-care allergy diagnostic device. The results shown in this paper present the use of the LIF-method for concentration calibration and the analysis of drying and resolving dyed proteins. The method is established successfully and delivers precise results.

【 授权许可】

Unknown