Insoluble-Bound Polyphenols Released from Guarana Powder: Inhibition of Alpha-Glucosidase and Proanthocyanidin Profile

Molecules

AdrianoCosta de Camargo¹  Fereidoon Shahidi²  GeniRodrigues Sampaio³  AnaClara da Costa Pinaffi³  ElizabethA. F. S. Torres³  MaiaraJurema Soares³ 

[1] Departamento de Ciencias Vegetales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad, Católica de Chile, Casilla 306-22, Santiago, Chile;Department of Biochemistry, Memorial University of Newfoundland, St. John’s, NL A1B 3X9, Canada;School of Public Health, University of Sao Paulo, 715 Dr Arnaldo Avenue, Sao Paulo, Sao Paulo 01246-904, Brazil;

关键词: paullinia cupana;    polyphenols;    proanthocyanidins;    alpha-glucosidase;    bioactive compounds;    type 2 diabetes;   

DOI  :  10.3390/molecules25030679

来源: DOAJ

【 摘 要 】

The Brazilian Food Supplement Law recently recognized that guarana (Paullinia cupana) contains bioactive substances, hence supporting its role as a functional food ingredient. The health benefits of guarana are associated, at least in part, to its phenolic compounds. However, to the best of our knowledge, there is no literature addressing the presence of phenolic compounds in the fraction containing insoluble-bound compounds and its contribution in terms of alpha-glucosidase inhibition. The concentration of phenolic extracts released from the insoluble-bound fraction required to inhibit 50% of alpha-glucosidase (IC₅₀) activity was 5.8-fold lower than that present in the soluble counterpart. Both fractions exhibited a mixed inhibition mode. Fourteen proanthocyanidins (dimers to tetramers) present in the insoluble-bound fraction were tentatively identified by MALDi-TOF-MS. Future studies aiming at increasing the concentration of the soluble counterpart are deemed necessary. The results presented here enhance the phenolic database of guarana and have a practical impact on the procurement of nutraceuticals and functional ingredients related to the prevention and/or management of type 2 diabetes. The Brazilian normative on food supplements has been recently revised. This study lends support to the future inclusion of guarana powder in the list of sources of proanthocyanidins for the industry of food supplements.

【 授权许可】

Unknown