Identification of Blueberry miRNAs and Their Targets Based on High-Throughput Sequencing and Degradome Analyses

International Journal of Molecular Sciences

Xiaoming Lou¹  Guangping Li²  Hailing Li³  Yun Wang⁴  Changqing Zhang⁴ 

[1] [e-mail](Y.W.);College of Forest, Nanjing Forestry University, Nanjing 210037, China;College of Horticultural Technology, Suzhou Polytechnic Institute of Agriculture, Suzhou 215008, China;College of Horticulture, Jinling Institute of Technology, Nanjing 210038, China;

关键词: blueberry;    miRNAs;    RNA-Seq;    gene expression;    degradome analysis;   

DOI  :  10.3390/ijms19040983

来源: DOAJ

【 摘 要 】

miRNAs are important regulators of plant gene expression. To better characterize their functions, we applied high-throughput sequencing and degradome analyses to investigate three blueberry (Vaccinium ashei) tissues. A total of 127 known and 101 novel miRNAs were identified. Moreover, 141 targets for 42 known and 19 novel miRNAs were experimentally validated by degradome sequencing. A functional analysis of these miRNA targets revealed they were associated with diverse biological activities and several pathways, e.g., anthocyanin biosynthesis and cytokinin signal transduction. The data presented herein expand our understanding of the regulation of blueberry miRNAs during floral and fruit development stages. They may also provide new insights into the roles of miRNAs during anthocyanin biosynthesis in blueberry fruits.

【 授权许可】

Unknown