Microbiological assessment of freshly-shot wild boars meat in Lazio Region, Viterbo territory: a preliminary study

Italian Journal of Food Safety

Enrica Martini¹  Massimo Palazzetti¹  Giuseppe Micarelli¹  Stefano Bilei²  Francesco Tomassetti²  Paola De Santis²  Alberto Brozzi²  Eda Maria Flores Rodas²  Teresa Bossù²  Tatiana Bogdanova²  Rita Tolli²  Sara Greco²  Sabrina Pecchi²  Roberto Condoleo² 

[1] Azienda Sanitaria Locale 9 - Sezione di Viterbo;Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Roma;

关键词: Wild boars, Foodborne pathogens, Salmonella spp., Yersinia enterocolitica, Escherichia coli;   

DOI  :  10.4081/ijfs.2014.1711

来源: DOAJ

【 摘 要 】

During the wild boars hunting season (2012-2013), 6508 adults were shot in Lazio Region, Viterbo territory. Out of the 6508, 393 subjects were sampled to be tested for the detection of Salmonella spp., Yersinia enterocolitica, E. coli VTEC and Trichinella spp. For this purpose, diaphragm and skeletal muscles samples were collected after the shooting, and tested at the Istituto Zooprofilattico Sperimentale Lazio and Toscana laboratories, Rome, Italy. Samples were tested respectively by polymerase chain reaction, enzyme linked immunofluorescent assay, microbiological and parasitological assays. The positivity percentages are 3.6% for Salmonella spp., 14.8% (out of 230 samples tested) for Yersinia enterocolitica, 4.6% for E.coli VTEC (vtx 1 and 2 genes). Each positive sample for vtx 1 and 2 was tested to isolate Verotoxigenic E.coli strains; none of them was positive for the same virulence genes. All diaphragm samples were negative to the trichinoscopic test. Every bacterial strain was identified serologically by seroagglutination test.

【 授权许可】

Unknown