Characterization of high risk human papilloma virus (HPV) genotypes associated with oropharyngeal cancers in a Nigerian population

The Pan African Medical Journal

Pam Luka¹  Tolulope Afolaranmi²  Ambrose Elugbe³  Samuel Agida Adoga⁴  Atiene Solomon Sagay⁵  Godwin Eremwan Imade⁵  Olugbenga Silas⁶  Helen Oluwadamilola Akhiwu⁷  Patrick Oladele Olorunfemi⁸  Patricia Odumosu⁹  Benjamin Tagbo Ugwu^1,10  Acheng Shedrach^1,11  Akinola Ladeinde^1,12  Nyam Chuwang^1,13  Benjamin Idemudia Akhiwu^1,14 

[1] Biotechnology Center, NVRI, Vom, Jos, Plateau State, Nigeria;Department of Community Medicine, University of Jos, Jos University Teaching Hospital, Jos, Plateau State, Nigeria;Department of Dental and Maxillofacial surgery, Jos University Teaching Hospital, Jos, Plateau State, Nigeria;Department of Ear, Nose and Throat surgery, University of Jos, Jos University Teaching Hospital, Lamingo Permanent Site, Jos, Plateau State, Nigeria;Department of OBGYN, Faculty of Clinical Sciences, College of Health Sciences, University of Jos, Jos, Plateau State, Nigeria;Department of Pathology, University of Jos, Jos University Teaching Hospital, Lamingo Permanent Site, Jos, Plateau State, Nigeria;Department of Pediatrics, Jos University Teaching Hospital, Lamingo Permanent Site, Jos, Plateau State, Nigeria;Department of Pharm, Microbiology and Biotechnology, University of Jos, Jos, Plateau State, Nigeria;Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Jos, Jos, Plateau State, Nigeria;Department of Surgery, University of Jos, Jos University Teaching Hospital, Lamingo Permanent Site, Jos, Plateau State, Nigeria;Genomics and Postgraduate Research Laboratory, College of Health Sciences, University of Jos, Jos, Plateau State, Nigeria;Oral and Maxillofacial Surgery Department, College of Medicine, University of Lagos, Lagos State, Nigeria;STAMINA Genomics Laboratory, Department of Human Anatomy, Faculty of Basic Medical sciences, University of Jos, Jos, Plateau State, Nigeria;Oral and Maxillofacial Surgery Department, University of Jos, Jos University Teaching Hospital, Lamingo permanent Site, Jos, Plateau State, Nigeria;

关键词: human papilloma virus;    nigeria;    oropharyngeal carcinoma;   

DOI  :  10.11604/pamj.2021.38.40.27309

来源: DOAJ

【 摘 要 】

INTRODUCTION: Head and neck cancers have essentially been a disease of the elderly but recent studies are beginning to demonstrate their increasing incidence in young people with infections such as human papilloma virus (HPV). This study was carried out to determine the prevalence of high risk Human papilloma virus (hrHPV) related oropharyngeal carcinoma and its prevalent genotypes as well as their strength of association with HIV in adult Nigerian subjects. METHODS: this was a cross-sectional study of 41 patients with oropharyngeal carcinomas seen over a 2-year period. Patients had incisional and/or excisional biopsy done under anesthesia. A portion of the specimen from which the DNA was extracted was placed in Digene HC2 DNA collection device while the 2nd portion for histopathological analysis was fixed using 10% Neutral Buffered Formalin (NBF) and embedded in paraffin blocks. Oropharyngeal cancer HPV genotyping was done using HPV genotypes 14 real-tm quant kit (SACACE, Italy). The data was analyzed using SPSS version 23. RESULTS: prevalence of HPV was 17.1% with a male to female ratio of 2.7:1. The identified genotypes were 16,33,35 and 52 with 28.6% of patients having more than one genotype. Most of the age groups studied were affected. Squamous cell carcinoma and Ameloblastic carcinoma were the cancers associated with HPV. HPV was not identified in the HIV positive patients. CONCLUSION: high-risk human papilloma virus genotypes 16,33, 35 and 52 are associated with oropharyngeal carcinoma in Nigeria but were not found in HIV patients. This finding provides a strong evidence for the use of the 9-valent prophylactic vaccine for the prevention of oropharyngeal cancer in Nigeria. Public awareness and HPV prevention strategies should reduce significantly the incidence of oropharyngeal carcinomas in our environment.

【 授权许可】

Unknown