Comprehensive annotations of human herpesvirus 6A and 6B genomes reveal novel and conserved genomic features

eLife

Roni Winkler¹  Yaara Finkel¹  Martina Dobesova¹  Michal Schwartz¹  Noam Stern-Ginossar¹  Julie Tai-Schmiedel¹  Aharon Nachshon¹  Dominik Schmiedel²  Ofer Mandelboim² 

[1] Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel;The Lautenberg Center for General and Tumor Immunology, Institute for Medical Research Israel-Canada, The Hebrew University Hadassah Medical School, Jerusalem, Israel;

关键词: human herpesvirus 6;    cytomegalovirus;    ribosome profiling;    genome annotations;    lncRNA;   

DOI  :  10.7554/eLife.50960

来源: DOAJ

【 摘 要 】

Human herpesvirus-6 (HHV-6) A and B are ubiquitous betaherpesviruses, infecting the majority of the human population. They encompass large genomes and our understanding of their protein coding potential is far from complete. Here, we employ ribosome-profiling and systematic transcript-analysis to experimentally define HHV-6 translation products. We identify hundreds of new open reading frames (ORFs), including upstream ORFs (uORFs) and internal ORFs (iORFs), generating a complete unbiased atlas of HHV-6 proteome. By integrating systematic data from the prototypic betaherpesvirus, human cytomegalovirus, we uncover numerous uORFs and iORFs conserved across betaherpesviruses and we show uORFs are enriched in late viral genes. We identified three highly abundant HHV-6 encoded long non-coding RNAs, one of which generates a non-polyadenylated stable intron appearing to be a conserved feature of betaherpesviruses. Overall, our work reveals the complexity of HHV-6 genomes and highlights novel features conserved between betaherpesviruses, providing a rich resource for future functional studies.

【 授权许可】

Unknown