Reduction in the Dietary VA Status Prevents Type 2 Diabetes and Obesity in Zucker Diabetic Fatty Rats

Biomolecules

Xia Tang¹  Jing Wang²  Xinge Hu³  Guoxun Chen³  Tiannan Wang³ 

[1] College of Food Science and Technology, Hebei Agriculture University, Baoding 071001, China;College of Pharmacy, South-Central University for Nationalities, Wuhan 430074, China;Department of Nutrition, University of Tennessee at Knoxville, Knoxville, TN 37996, USA;

关键词: vitamin A;    type 2 diabetes;    Zucker diabetic fatty rats;    oral glucose tolerance test;    blood glucose;    hepatic protein expression;   

DOI  :  10.3390/biom12040528

来源: DOAJ

【 摘 要 】

We hypothesized that the vitamin A (VA) status regulates type 2 diabetes (T2D) development in Zucker diabetic fatty (ZDF) rats. Zucker Lean and ZDF rats at weaning were fed a VA deficient with basal fat (VAD-BF, no VA and 22.1% fat energy), VA marginal with BF (VAM-BF, 0.35 mg retinyl palmitate (RP)/kg), VA sufficient with BF (VAS-BF, 4.0 mg RP/kg), VAD with high fat (VAD-HF, 60% fat energy), VAM-HF or VAS-HF diet for 8 weeks, including an oral glucose tolerance test (OGTT) at week 7.5. The hepatic mRNA and proteins levels were determined using real-time PCR and Western blot, respectively. The VAD-BF/HF and VAM-BF/HF diets prevented peripheral hyperglycemia and attenuated obesity in ZDF rats, which occurred in the presence of the VAS-BF/HF diets. This lowered VA status reduced venous blood hyperglycemia, hyperinsulinemia and hyperlipidemia, and improved OGTT and homeostasis model assessment for insulin resistance results in ZDF rats. The expression levels of key hepatic genes for glucose and fat metabolism were regulated by VA status and dietary fat contents. An interaction between VA and HF condition was also observed. We conclude that the reduction in the dietary VA status in both BF and HF conditions prevents T2D and obesity in ZDF rats.

【 授权许可】

Unknown