High-quality draft genome sequence of Ensifer meliloti Mlalz-1, a microsymbiont of Medicago laciniata (L.) miller collected in Lanzarote, Canary Islands, Spain

Standards in Genomic Sciences

Victor Markowitz¹  Amrita Pati¹  Margaret Gollagher²  Tanja Woyke³  Rekha Seshadri³  Natalia Ivanova³  Nikos Kyrpides³  T. B. K. Reddy³  Milagros León-Barrios⁴  Encarna Velázquez⁵  Mohamed N. Baeshen⁶  Naseebh Nabeeh Baeshen⁶  Julie Ardley⁷  Rui Tian⁷  Wayne Reeve⁷  Wan Adnawani Meor Osman⁷  Patrick Elia⁸  Peter van Berkum⁸ 

[1] Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory;Curtin University Sustainability Policy Institute, Curtin University;DOE Joint Genome Institute;Departamento de Bioquímica, Microbiología, Biología Celular y Genética, Universidad de La Laguna;Departamento de Microbiología y Genetica and Instituto Hispanoluso de Investigaciones Agrarias (CIALE), Universidad de Salamanca;Department of Biology, Faculty of Science, University of Jeddah;School of Veterinary and Life Sciences, Murdoch University;U.S. Department of Agriculture, Soybean Genomics and Improvement Laboratory, Beltsville Agricultural Research Center;

关键词: Root-nodule bacteria;    Ensifer;    Geba-Rnb;    Medicago;    lpiA-acvB operon;   

DOI  :  10.1186/s40793-017-0270-2

来源: DOAJ

【 摘 要 】

Abstract Ensifer meliloti Mlalz-1 (INSDC = ATZD00000000 ) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from an effective nitrogen-fixing nodule of Medicago laciniata (L.) Miller from a soil sample collected near the town of Guatiza on the island of Lanzarote, the Canary Islands, Spain. This strain nodulates and forms an effective symbiosis with the highly specific host M. laciniata. This rhizobial genome was sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) sequencing project. Here the features of E. meliloti Mlalz-1 are described, together with high-quality permanent draft genome sequence information and annotation. The 6,664,116 bp high-quality draft genome is arranged in 99 scaffolds of 100 contigs, containing 6314 protein-coding genes and 74 RNA-only encoding genes. Strain Mlalz-1 is closely related to Ensifer meliloti IAM 12611 T, Ensifer medicae A 321T and Ensifer numidicus ORS 1407 T, based on 16S rRNA gene sequences. gANI values of ≥98.1% support the classification of strain Mlalz-1 as E. meliloti . Nodulation of M. laciniata requires a specific nodC allele, and the nodC gene of strain Mlalz-1 shares ≥98% sequence identity with nodC of M. laciniata-nodulating Ensifer strains, but ≤93% with nodC of Ensifer strains that nodulate other Medicago species. Strain Mlalz-1 is unique among sequenced E. meliloti strains in possessing genes encoding components of a T2SS and in having two versions of the adaptive acid tolerance response lpiA-acvB operon. In E. medicae strain WSM419 , lpiA is essential for enhancing survival in lethal acid conditions. The second copy of the lpiA-acvB operon of strain Mlalz-1 has highest sequence identity (> 96%) with that of E. medicae strains, which suggests genetic recombination between strain Mlalz-1 and E. medicae and the horizontal gene transfer of lpiA-acvB.

【 授权许可】

Unknown