The Use of Hyperimmune Chicken Reference Sera Is Not Appropriate for the Validation of Influenza Pseudotype Neutralization Assays

Pathogens

Giovanni Cattoli¹  Simon Scott²  Nigel Temperton²  Eleonora Molesti²  Francesca Ferrara² 

[1] Animal Production and Health Laboratory, Joint FAO/IAEA Division, International Atomic Energy Agency, A-2444 Seibersdorf, Austria;Viral Pseudotype Unit, Medway School of Pharmacy, University of Kent, Chatham, Kent ME4 4TB, UK;

关键词: influenza;    serology;    standards;    validation;    pseudotypes;    neutralization;   

DOI  :  10.3390/pathogens6040045

来源: DOAJ

【 摘 要 】

The pseudotype particle neutralization test (pp-NT) is a next-generation serological assay employed for the sensitive study of influenza antibody responses against hemagglutinin (HA), including stalk-directed antibodies. However, a validation of this assay has yet to be performed, and this limits its use to primarily research laboratories. To identify possible serological standards to be used in optimization and validation of the pp-NT, we have evaluated the cross-reactivity of hyperimmune chicken reference antisera in this assay. Our findings show that the cross-reactivity detected by the pp-NT is only partly explained by phylogenetic relationships and protein homology between the HA subtypes analysed; further studies are necessary to understand the origin of the cross-reactivity detected, and reference standards with higher specificity should be evaluated or generated de novo for future use in pp-NT.

【 授权许可】

Unknown