Regulation of glucose and lipid metabolism by insulin and glucagon in vivo and in vitro in common carp Cyprinus carpio L.

Aquaculture Reports

Chaobin Qin¹  Guoxing Nie²  Dapeng Deng³  Ronghua Lu³  Xiao Yan³  Guiqin Wang³  Junchang Feng³  Guokun Yang³ 

[1] College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agriculture University, Changchun, Jilin, 130118, China;College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agriculture University, Changchun, Jilin, 130118, China;College of Fisheries, Henan Normal University, Xinxiang, Henan, 453000, China;

关键词: Cyprinus carpio L.;    Insulin;    Glucagon;    Glucose metabolism;    Lipid metabolism;   

DOI  :  

来源: DOAJ

【 摘 要 】

Our objective is to study the low metabolic utilization of dietary carbohydrates in fish. The regulation of key enzymes involved in glucose and lipid metabolism in the common carp (Cyprinus carpio L.), known to be relatively tolerant to dietary carbohydrates, were assessed. We performed intraperitoneal (i.p.) administration of insulin and glucagon to common carp and also subjected common carp primary hepatocytes to insulin or glucagon. The results showed that insulin administration induced hypoglycaemia through up-regulation of the expression of the glycolysis genes glucokinase (GK) and hexose kinase (HK), coupled with increased gene expression of fatty acid synthase (FAS), acetyl-CoA carboxylase-1 (ACC-1), sterol-regulator element-binding protein-1 (SREBP-1). Insulin also limited the expression of the gluconeogenesis genes phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), coupled with decreased gene expression of lipoprotein lipase (LPL) and carnitine palmitoyl transferase I (CPT-1). However, glucagon had the opposite effect. The results showed that glucagon induced hyperglycemia with increased expression of PEPCK and G6Pase and increased lipolytic capacity as indicated by lower mRNA levels of SREBP-1. Glucagon (i.p.) significantly increased the activity of PEPCK and LPL in hepatopancreas. In addition, the mRNA expression of HK showed an up-regulated trend and the mRNA expression of PEPCK and G6Pase showed a down-regulated trend in the group of glucose (i.p.). This study demonstrates that insulin and glucagon possess the intrinsic ability to regulate hepatic energy metabolism in common carp, the regulation of which seems to be similar to that of the mammals at least at the molecular level.

【 授权许可】

Unknown