The Inhibitory Mechanisms Study of 5,6,4′-Trihydroxy-7,3′-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability

Molecules

Guan-Jhong Huang¹  Chia-Hua Liang²  Shin-Hun Juang³  Fong-Pin Liang³  Hsiou-Yu Ding⁴  Wen-Chuan Lin⁵  Shih-Hao Wang⁵  Shiuan-Pey Lin⁵ 

[1] Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan;Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan;Department of Pharmacy, Tajen University, Pingtung 907, Taiwan;Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan;School of Pharmacy, China Medical University, Taichung 404, Taiwan;

关键词: Anisomeles ovata;    5,6,4′-trihydroxy-7,3′-dimethoxyflavone;    anti-inflammatory;    anti-oxidant;    reactive oxygen species;   

DOI  :  10.3390/molecules21020136

来源: DOAJ

【 摘 要 】

The whole plant of Anisomeles ovata has been widely used in Taiwan for treating inflammation-related skin and liver diseases, however, the detailed pharmacology mechanisms have yet to be elucidated. In the present study, one of the major components, 5,6,4′-trihydroxy-7,3′-dimethoxyflavone (5-TDMF), was purified from a methanol extract of Anisomeles ovata. A pharmacological study of this compound suggests that 5-TDMF possesses potent free radical scavenging activity both in vitro and ex vivo. Furthermore, 5-TDMF reduces nitric oxide and pro-inflammatory cytokine production in LPC-treated RAW 264.7 cells through the attenuation of nitric oxide synthase and cyclooxygenase-2. Additional experiments suggest that of 5-TDMF interferes with nuclear factor-κB translocation and mitogen-activated protein kinase pathways. These results identify 5-TDMF as an anti-oxidant and anti-inflammatory compound, explain the pharmacologic function of Anisomeles ovata and suggest its great potential as a new anti-inflammatory remedy.

【 授权许可】

Unknown