Molecular Characterisation of Soybean Osmotins and Their Involvement in Drought Stress Response

Frontiers in Genetics

Paula Bacaicoa Caruso¹  Frank Lino Guzman²  Luis Fernando Saraiva Macedo Timmers³  Osmar Norberto de Souza³  Débora Favero⁴  Christian Bredemeier⁴  Maria Helena Bodanese-Zanettini⁵  Luisa Abruzzi de Oliveira-Busatto⁵  Giulia Ramos Faillace⁵  Ciliana Rechenmacher⁵ 

[1] Laboratório de Bioinformática, Modelagem e Simulação de Biossistemas (LABIO), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil;Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil;Programa de Pós-Graduação em Biologia Celular e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil;Programa de Pós-Graduação em Fitotecnia, Departamento de Plantas de Lavoura, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil;Programa de Pós-Graduação em Genética e Biologia Molecular and Instituto Nacional de Ciência e Tecnologia: Biotec Seca-Pragas, Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil;

关键词: phylogenetic analysis;    gene structure;    chromosomal localisation;    protein structure;    gene expression;    response to dehydration;   

DOI  :  10.3389/fgene.2021.632685

来源: DOAJ

【 摘 要 】

Osmotins are multifunctional proteins belonging to the thaumatin-like family related to plant stress responses. To better understand the functions of soybean osmotins in drought stress response, the current study presents the characterisation of four previously described proteins and a novel putative soybean osmotin (GmOLPa-like). Gene and protein structure as well as gene expression analyses were conducted on different tissues and developmental stages of two soybean cultivars with varying dehydration sensitivities (BR16 and EMB48 are highly and slightly sensitive, respectively). The analysed osmotin sequences share the conserved amino acid signature and 3D structure of the thaumatin-like family. Some differences were observed in the conserved regions of protein sequences and in the electrostatic surface potential. P21-like present the most similar electrostatic potential to osmotins previously characterised as promoters of drought tolerance in Nicotiana tabacum and Solanum nigrum. Gene expression analysis indicated that soybean osmotins were differentially expressed in different organs (leaves and roots), developmental stages (R1 and V3), and cultivars in response to dehydration. In addition, under dehydration conditions, the highest level of gene expression was detected for GmOLPa-like and P21-like osmotins in the leaves and roots, respectively, of the less drought sensitive cultivar. Altogether, the results suggest an involvement of these genes in drought stress tolerance.

【 授权许可】

Unknown