The Effect ofDifferent Orthodontic Forces on MMP 9 Expression in a RatDiabetic Model

Journal of Tropical Life Science

SriWidyarti¹  Nur Permatasari²  Hafiedz Maulana³  Amandia Dewi Permana Shita³  Nuzulul Hikmah³ 

[1] Biology Department, Mathematics and Natural Sciences Faculty,Brawijaya University,Malang- Indonesia;Laboratory of Pharmacology, Medical Faculty, Brawijaya University, Malang- Indonesia;Master Program of Biomedic Science, Medical Faculty, Brawijaya University, Malang- Indonesia;

关键词: Orthodontic force;    diabetes;    MMP 9;    fibroblast;    remodeling;   

DOI  :  

来源: DOAJ

【 摘 要 】

Orthodonticforcesproducealveolarboneandperiodontalligamentremodelingthatcausetooth movement. Diabetes can increase tissue damage and, therefore, contributes to the process of orthodontictoothmovement.Theaimofthisstudywastodeterminetheeffectofdifferently orthodontic forces on the expression of MMP nine in rat diabetic model. Twenty four male Wistar rats weredividedintothreegroupsofcontrolandthreegroupsoftreatmentstreptozotocin-induced diabetes with stratified doses. Application of orthodontic appliance was performed with different forces (10,20and30grF).Orthodonticappliancewasperformedonbothupperincisors.Immunohistochemical(IHC)analysiswasusedtoobservetheexpressionofMMP9andHE(HematoxylenEosin) stainingtoobservethenumberoffibroblasts.Statisticalanalysiswascarriedoutusingt-testand SpearmancorrelationanalysistodeterminethedifferenceofMMPnineexpressionandnumberof fibroblastsbetweengroups,anddeterminethecorrelationofboth.Theresultsshowedanincreased expressionofMMP9anddecreasedoffibroblastsnumberindiabeticrats,alongwithincreasing magnitudeoforthodonticforces.Thedifferentorthodonticforcesgiventodiabeticratsaffectthe expressionofMMP9andthenumberoffibroblasts,inwhichtheexpressionofMMP9increasedalong with the increase of orthodontic forces, both at pressure and tension sides.

【 授权许可】

Unknown