期刊论文详细信息
Molecules
Effect of Pentacyclic Triterpenoids-Rich Callus Extract of Chaenomeles japonica (Thunb.) Lindl. ex Spach on Viability, Morphology, and Proliferation of Normal Human Skin Fibroblasts
Anna Stochmal1  Jerzy Żuchowski1  Małgorzata Kotwicka2  Małgorzata Chmielewska2  MałgorzataAnna Kikowska3  Agata Włodarczyk3  Barbara Thiem3  Elżbieta Studzińska-Sroka4 
[1] Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland;Department of Cell Biology, Poznan University of Medical Sciences, 60-806 Poznań, Poland;Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 61-861 Poznań, Poland;Department of Pharmacognosy, Poznan University of Medical Sciences, 60-781 Poznań, Poland;
关键词: Chaenomeles japonica;    polyphenolics;    pentacyclic triterpenes;    antioxidant activity;    in vitro cultures;    skin fibroblasts;   
DOI  :  10.3390/molecules23113009
来源: DOAJ
【 摘 要 】

The effect of the well-characterized callus extract of Chaenomeles japonica on viability, morphology, and proliferation of normal human skin fibroblasts was investigated. The phytochemical analysis was performed using the ultra-high performance liquid chromatography method. The total phenolic, phenolic acid, and flavonoid contents were determined spectrophotometrically. The antioxidant activity was investigated using the DPPH (1,1-Diphenyl-1-picrylhydrazyl Radical Scavenging), FRAP (Ferric Reducing Antioxidant Power), and CUPRAC (CUPric Reducing Antioxidant Capacity) assays. The callus growth index during passages was high as well as the content of pentacyclic triterpenoids. The microscopic observations of the fibroblast viability, morphology and the evaluation of the proliferation ratio (xCELLigence system) proved that the influence of callus extract on the fibroblasts was dose-dependent. The evaluated level of fibroblasts proliferation rate after 72 h of incubation with callus extract at concentration 12.5 µg L−1 was the highest compared to all the analyzed ligands. Moreover, callus extract administrated for 72 h caused a significant increase in the proliferation rate in comparison with the control group (5.7 ± 0.1 vs. 4.4 ± 0.9; p < 0.01). The preliminary studies carried out may suggest that the callus extract rich in triterpenoids may be a potential source of cosmetic ingredients with a beneficial effect on human skin.

【 授权许可】

Unknown   

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