Haplotype block analysis of an Argentinean hexaploid wheat collection and GWAS for yield components and adaptation

BMC Plant Biology

William John Rogers¹  Silvana Marisol Luján Basile¹  Melina Demichelis²  Marcelo Helguera²  Juan Manuel Crescente²  Maria Belén Conde²  Leonardo Sebastián Vanzetti²  Ana Clara Pontaroli³  Pablo Abbate³  Ignacio Abel Ramírez³ 

[1] Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-INBIOTEC-CONICET, Facultad de Agronomía, UNCPBA.;Laboratorio de Biotecnología, EEA INTA Marcos Juárez, Grupo Biotecnología y Recursos Genéticos, Instituto Nacional de Tecnología Agropecuaria;Unidad Integrada Balcarce Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata - Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología;

关键词: Wheat;    Haplotype;    GWAS;    Heading date;    Plant height;    Thousand grain weight;   

DOI  :  10.1186/s12870-019-2015-4

来源: DOAJ

【 摘 要 】

Abstract Background Increasing wheat (Triticum aestivum L.) production is required to feed a growing human population. In order to accomplish this task a deeper understanding of the genetic structure of cultivated wheats and the detection of genomic regions significantly associated with the regulation of important agronomic traits are necessary steps. To better understand the genetic basis and relationships of adaptation and yield related traits, we used a collection of 102 Argentinean hexaploid wheat cultivars genotyped with the 35k SNPs array, grown from two to six years in three different locations. Based on SNPs data and gene-related molecular markers, we performed a haplotype block characterization of the germplasm and a genome-wide association study (GWAS). Results The genetic structure of the collection revealed four subpopulations, reflecting the origin of the germplasm used by the main breeding programs in Argentina. The haplotype block characterization showed 1268 blocks of different sizes spread along the genome, including highly conserved regions like the 1BS chromosome arm where the 1BL/1RS wheat/rye translocation is located. Based on GWAS we identified ninety-seven chromosome regions associated with heading date, plant height, thousand grain weight, grain number per spike and fruiting efficiency at harvest (FEh). In particular FEh stands out as a promising trait to raise yield potential in Argentinean wheats; we detected fifteen haplotypes/markers associated with increased FEh values, eleven of which showed significant effects in all three evaluated locations. In the case of adaptation, the Ppd-D1 gene is consolidated as the main determinant of the life cycle of Argentinean wheat cultivars. Conclusion This work reveals the genetic structure of the Argentinean hexaploid wheat germplasm using a wide set of molecular markers anchored to the Ref Seq v1.0. Additionally GWAS detects chromosomal regions (haplotypes) associated with important yield and adaptation components that will allow improvement of these traits through marker-assisted selection.

【 授权许可】

Unknown