Salidroside and FG-4592 ameliorate high glucose-induced glomerular endothelial cells injury via HIF upregulation

Biomedicine & Pharmacotherapy

Tong-xia Cui¹  Hamze Ibrahim Rage²  Qiao-lan He²  Xue-ling Fang²  Wei-ping Zhu²  Rui-yan Xie²  Wei-ze Lv³  Xiao-bin Zheng⁴  Yi-jie Li⁵ 

[1] Corresponding authors.;Department of Nephrology, the Fifth Affiliated Hospital of Sun Yat-sen University, No.52 Meihua Road, Zhuhai, 519000, China;Department of Oncology, the Fifth Affiliated Hospital of Sun Yat-sen University, No.52 Meihua Road, Zhuhai, 519000, China;Department of Respiratory Medicine, the Fifth Affiliated Hospital of Sun Yat-sen University, No.52 Meihua Road, Zhuhai, 519000, China;Second Department of General Surgery, the Fifth Affiliated Hospital of Sun Yat-sen University, No.52 Meihua Road, Zhuhai, 519000, China;

关键词: FG-4592;    HIF-1α;    HIF-2α;    High glucose;    Salidroside;   

DOI  :  

来源: DOAJ

【 摘 要 】

Increasing research indicates that hyperglycemia plays a crucial role in the progression of diabetic nephropathy (DN); however, effective treatment for preventing or slowing DN progression are seriously lacking. Although salidroside (SAL) has been demonstrated to have a positive anti-diabetic effect, the cellular mechanisms remain unclear. FG-4592, a novel prolyl hydroxylase inhibitor, was used to regulate HIF-1α and HIF-2α expression. The present study aimed to explore the underlying mechanisms of SAL and FG-4592 on high glucose (HG)-induced rat glomerular endothelial cells (rGECs) injury. HG-cultured rGECs were used to induce a diabetic environment. An MTT assay, RT-qPCR, Western blot, flow cytometry, and immunofluorescent staining were performed to investigate the effects of SAL on HG-induced rGECs injury. FG-4592 and SAL protected rGECs against HG-induced injury by increasing cellular viability and reducing the cell apoptosis rate. SAL and FG-4592 downregulated PHD-2 expression and upregulated HIF-1α and HIF-2α expression. In conclusion, our findings suggest that SAL and FG-4592 ameliorate HG-induced rGEC injury by upregulating HIF expression, indicating that SAL and FG-4592 might be favorable for further DN-treatment.

【 授权许可】

Unknown