A Novel Prebiotic Blend Product Prevents Irritable Bowel Syndrome in Mice by Improving Gut Microbiota and Modulating Immune Response

Nutrients

Yiping Ren¹  Jihong Lu¹  Lei Chen²  Chang Liu²  Xiaokui Guo²  Yan Zhang²  Qian Chen²  Mark Bartlett³ 

[1] Center for Anti-Aging Research, Nu Skin Enterprises, Shanghai 201401, China;Department of Microbiology and Immunology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;Nu Skin Enterprises Anti-Aging Research Center, Provo, UT 84601, USA;

关键词: prebiotics;    irritable bowel syndrome;    inflammation;    visceral hypersensitivity;    gut microbiota;    prebiotic blend (PB);   

DOI  :  10.3390/nu9121341

来源: DOAJ

【 摘 要 】

Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder yet it still lacks effective prevention therapies. The aim of this study is to determine whether a novel prebiotic blend (PB) composed of fructo-oligosaccharide (FOS), galactooligosaccharide (GOS), inulin and anthocyanins could be effective in preventing the development of IBS. We explored the possible mechanisms both in animal and in cells. Post-infectious IBS models in C57BL/6 mice were established and were pretreated with the PB, PB and probiotic strains 8 weeks in advance of infection. Eight weeks after infection, intestinal tissues were collected for assessing histomorphology, visceral sensitivity, barrier function, pro-inflammatory cytokines expression and proteomics analysis. Fecal samples were also collected for microbiota analysis. The pro-inflammatory cytokines expression in Caco-2 cells were evaluated after co-incubation with PB and Salmonella typhimurium 14028. The results showed that PB significantly decreased the pro-inflammatory cytokines both in infected Caco-2 cells and PI-IBS models. The loss of body weight, decreased expression of tight junction protein Occludin (OCLN), and changes of the microbiota composition induced by infections could be greatly improved by PB intervention (p < 0.05). The proteomics analysis revealed that this function was associated with Peroxisome proliferator-activated receptor (PPAR)γ pathway.

【 授权许可】

Unknown