pH-Controlled fluorescence switching in water-dispersed polymer brushes grafted to modified boron nitride nanotubes for cellular imaging

Beilstein Journal of Nanotechnology

Saban Kalay¹  Mustafa Çulha¹  Volodymyr Donchak²  Yurij Stetsyshyn²  Stanislav Voronov²  Yuriy Panchenko²  Ostap Lishchynskyi²  Khrystyna Harhay²  Halyna Ohar² 

[1] Department of Genetics and Bioengineering, Yeditepe University, Atasehir, 34755 Istanbul, Turkey;Lviv Polytechnic National University, 12 S. Bandery, 79013 Lviv, Ukraine;

关键词: boron nitride nanotubes;    cellular imaging;    fluorescence;    ph switching;    polymer brushes;    surface modification;   

DOI  :  10.3762/bjnano.10.233

来源: DOAJ

【 摘 要 】

pH-Switchable, fluorescent, hybrid, water-dispersible nanomaterials based on boron nitride nanotubes (BNNTs) and grafted copolymer brushes (poly(acrylic acid-co-fluorescein acrylate) – P(AA-co-FA)) were successfully fabricated in a two-step process. The functionalization of BNNTs was confirmed by spectroscopic, gravimetric and imaging techniques. In contrast to “pure” BNNTs, P(AA-co-FA)-functionalized BNNTs demonstrate intense green fluorescence emission at 520 nm. Under neutral or alkaline pH values, P(AA-co-FA)-functionalized BNNTs are highly emissive in contrast to acidic pH conditions where the fluorescent intensity is absent or low. No increase in the absorption was observed when the suspension pH was increased from 7 to 10. The functionalized BNNTs are easily taken up by human normal prostate epithelium (PNT1A) and human prostate cancer cell lines (DU145) and are suitable for further evaluation in cellular imaging applications.

【 授权许可】

Unknown