Rutin—Increased Antioxidant Activity and Skin Penetration by Nanocrystal Technology (smartCrystals)

Cosmetics

Cornelia M. Keck¹  Martina Meinke²  Rainer H. Müller³  Sung Min Pyo³ 

[1]Applied Pharmacy, University of Applied Sciences Kaiserslautern, Carl-Schurz-Str. 10-16, Pirmasens 66953, Germany

[2]Department of Dermatology, Venerology and Allergology, Charité-Universitätsmedizin Berlin, Charitéplatz 1, Berlin 10117, Germany

[3]Institute of Pharmacy, Pharmaceutics, Pharmaceutical Nanotechnology & NutriCosmetics, Kelchstr. 31, Berlin 12169, Germany

关键词: rutin;    nanocrystals;    smartCrystals;    poor solubility;    in vitro antioxidant activity;    DPPH assay;    radical scavenging;    ex vivo penetration study;    tape stripping;    bioactivity;   

DOI  :  10.3390/cosmetics3010009

来源: DOAJ

【 摘 要 】

Rutin is a well-known antioxidant from the group of flavonoids. Its use in cosmetic dermal products is, however, limited due to its poor water solubility. In order to increase rutin saturation solubility and improve the diffusion to the skin, rutin nanocrystals were produced by the smartCrystal process, e.g., bead milling followed by high pressure homogenization. Rutin nanocrystals were further incorporated into hydroxypropyl cellulose (HPC) gel and its long-term stability was assessed. Determination of the antioxidant activity was made by the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay for these formulations: rutin nanocrystals (mean size 300 nm), rutin raw drug powder (mean size 33 μm) and commercial product. Furthermore, the skin penetration profile of rutin was investigated by the tape-stripping method on porcine skin. This study demonstrated that rutin nanocrystal gel had the highest neutralizing activity (90%), followed by a commercial product and rutin raw drug powder. According to the skin study, rutin nanocrystals penetrated to the deeper layers of the stratum corneum, the horny layer of the skin.

【 授权许可】

Unknown