Enhancing thermal tolerance of Aspergillus niger PhyA phytase directed by structural comparison and computational simulation

BMC Biotechnology

Yunjuan Yang¹  Rui Zhang¹  Zunxi Huang¹  Qian Wu¹  Tingting Yu¹  Bo Xu¹  Huabiao Miao¹  Nanyu Han¹ 

[1] School of Life Sciences, Yunnan Normal University;

关键词: Phytase;    Thermostability;    Site-directed mutagenesis;    Homologous structural comparison;    Molecular dynamics simulation;   

DOI  :  10.1186/s12896-018-0445-y

来源: DOAJ

【 摘 要 】

Abstract Background Phytase supplied in feeds for monogastric animals is important for improving nutrient uptake and reducing phosphorous pollution. High-thermostability phytases are particularly desirable due to their ability to withstand transient high temperatures during feed pelleting procedures. A comparison of crystal structures of the widely used industrial Aspergillus niger PhyA phytase (AnP) with its close homolog, the thermostable Aspergillus fumigatus phytase (AfP), suggests 18 residues in three segments associated with thermostability. In this work, we aim to improve the thermostability of AnP through site-directed mutagenesis. We identified favorable mutations based on structural comparison of homologous phytases and molecular dynamics simulations. Results A recombinant phytase (AnP-M1) was created by substituting 18 residues in AnP with their AfP analogs. AnP-M1 exhibited greater thermostability than AnP at 70 °C. Molecular dynamics simulations suggested newly formed hydrogen bonding interactions with nine substituted residues give rise to the improved themostability. Thus, another recombinant phytase (AnP-M2) with just these nine point substitutions was created. AnP-M2 demonstrated superior thermostability among all AnPs at ≥70 °C: AnP-M2 maintained 56% of the maximal activity after incubation at 80 °C for 1 h; AnP-M2 retained 30-percentage points greater residual activity than that of AnP and AnP-M1 after 1 h incubation at 90 °C. Conclusions The resulting AnP-M2 is an attractive candidate in industrial applications, and the nine substitutions in AnP-M2 are advantageous for phytase thermostability. This work demonstrates that a strategy combining structural comparison of homologous enzymes and computational simulation to focus on important interactions is an effective method for obtaining a thermostable enzyme.

【 授权许可】

Unknown