The effects Metformin/Irinotecan-loaded PLGA nanoparticles on glutamate re-uptake time and alteration EAAT1 gene expression level in vitro

Nanomedicine Journal

Ahmet Hacimuftuoglu¹  Selcuk butuner¹  Maryam mohammadzadeh¹  Numan Taspinar¹  Meltem Cetin²  Afifie ugur²  Ali Taghizadehghalehjoughi³ 

[1] Department of Medical Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, 25240, Turkey;Department of Pharmaceutical Technology, Faculty of Pharmacy, Ataturk University, Erzurum, 25240, Turkey;Department of Pharmacology and Toxicology, Faculty of Veterinary, Ataturk University, Erzurum, 25240, Turkey;

关键词: EAAT1;    Irinotecan;    Metformin;    PLGA;    Voltammetry;   

DOI  :  10.22038/nmj.2019.06.005

来源: DOAJ

【 摘 要 】

Objective(s): The present study was designed to evaluate of Metformin/Irinotecan-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) effects on glutamate re-uptake time and receptor expression status in both glioblastoma multiforme (GBM) and cortex neuron cultures. The study was performed on glioblastoma cell line and primer cortex neuron.Materials and Methods: The re-uptake time and gene expression status of pure drugs and MET- or IRI-loaded-PLGA NPs on healthy neuron cells and U-87 MG cell line were investigated by using glutamate specific voltammetry electrodes technique and real time PCR. Results: Both MET and MET-PLGA NPs (1 and 2 mM) exhibited significant cytotoxicity on both U87MG and neuron cells. MET and MET-PLGA NPs (0.5 mM) showed lower cytotoxic effects on both cells. IRI and IRI-PLGA NPs (100 µM) had significant cytotoxic effects on both cell lines. Conclusion: All drug-loaded NPs caused a significant reduction in glutamate reuptake time compared with free drugs, blank NPs and cancer cells control groups. Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach to treat GBM.

【 授权许可】

Unknown