In vitro antiviral activity of BanLec against herpes simplex viruses type 1 and 2

Bangladesh Journal of Pharmacology

Ashish Wadhwani¹  Gowri Subramaniam²  Arisha Taj Mahaboob Batcha³ 

[1] Assistant Professor and Head, 2Department of Pharmaceutical Biotechnology, JSS Academy of Higher Education & Research, JSS College of Pharmacy, Ooty-643001, Tamil Nadu, India.;Professor, Department of Biochemistry,Dr.N.G.P Arts and Science College, Coimbatore, Tamil Nadu. – 641048,India;Research Scholar, Department of Biochemistry, Dr.N.G.P Arts and Science College, Coimbatore, Tamil Nadu.;

关键词: Antiviral;    BanLec;    Herpes simplex;    Musa paradisiaca;   

DOI  :  10.3329/bjp.v15i1.42320

来源: DOAJ

【 摘 要 】

The present study evaluates the antiviral activity of banana lectin (BanLec) against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2). Lectin was isolated from the ripen pulp of bananas (Musa paradisiaca). The study showed that lectin exhibited hemagglutination activity towards human erythrocytes A, B, AB and O group. The molecular weight of BanLec using SDS gel-electrophoresis was found to be 14,000-30,000 Da. Cytotoxicity of BanLec on the Vero cell lines showed an inhibitory concentration of 172.7 µg/mL. BanLec was virucidal and showed no cytotoxicity at the concentration tested. The lectin showed a dose-dependent antiviral activities, inhibiting HSV-1 by 16.0 µg/mL with selectivity index 10.8 and HSV-2 inhibition by 67.7 µg/mL with selectivity index 2.6. These results corroborate that BanLec could be a rich source of potential antiviral compound for HSV-1 when compared to HSV-2.

【 授权许可】

Unknown