Sargassum horneri ethanol extract containing polyphenols attenuates PM-induced oxidative stress via ROS scavenging and transition metal chelation

Journal of Functional Foods

Hyun Jung Kim¹  Hyun-Soo Kim²  Hyo Jin Kim³  Youngheun Jee⁴  You-Jin Jeon⁴  Kalahe Hewage Iresha Nadeeka Madushani Herath⁵  Duong Thi Thuy Dinh⁶ 

[1] National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101-gil, Janghang-eup, Seocheon, Republic of Korea;Science, Jeju National University, Jeju 63243, Republic of Korea;Department of Food Bioengineering, Jeju National University, Jeju 63243, Republic of Korea;Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea;Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea;;Interdisciplinary Graduate Program in Advanced Convergence Technology &

关键词: Particulate matter (PM);    MLE-12 cells;    Oxidative stress;    Metal chelating;    Antioxidant mechanisms;    Sargassum horneri;   

DOI  :  

来源: DOAJ

【 摘 要 】

Particulate matter (PM) generate oxidative stress and damage to exposed cells. In this study, we investigated the antioxidant mechanisms of Sargassum horneri ethanol extract (SHE) against PM in lung epithelial cell, MLE-12 cells. We discovered that transition metals in PM adhered to the surfaces of MLE-12 cells, triggering intracellular ROS generation and lipid peroxidation. The PM-induced oxidative stress in turn reduced the antioxidant enzymes activities. This disturbance in redox balance further produced oxidative DNA damage and eventually led to apoptotic cell death as expected. However, we uncovered that SHE containing polyphenols chelated the transition metals (Si, Mg, Al, Ti, and Fe) on the surfaces of MLE-12 cells. In addition, SHE not only attenuated the intracellular ROS and lipid peroxidation but also regulated antioxidant enzymes to suppress the PM-induce oxidative DNA damage and apoptotic cell death. These results suggest that SHE containing polyphenols contribute to the alleviation of the PM-induced oxidative stress and apoptotic cell death via ROS scavenging and transition metals chelating.

【 授权许可】

Unknown