Chemical modification of β-lactoglobulin by quinines

Journal of the Serbian Chemical Society

Božić Nataša M.¹  Božić Tatjana T.¹  Milosavić Nenad B.¹  Sladić Dušan M.¹  Vujčić Zoran M.¹  Novaković Irena² 

[1] Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, P. O. Box 158, 11001 Belgrade, Serbia and Montenegro;Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Njegoševa 12, P. O. Box 473, 11001 Belgrade;

关键词: avarone;    quinone;    β-lactoglobulin;    covalent modification.;   

DOI  :  10.2298/JSC0305243N

来源: DOAJ

【 摘 要 】

The avarone/avarol quinone/hydroquinone couple, as well as their derivatives show considerable antitumor activity. In this work, covalent modifications of β-lactoglobulin, isolated from cow milk, by avarone, its model compound 2-tert-butyl-1,4-benzoquinone, and several of their alkylthio derivatives were studied. The techniques applied for assaying the modifications were UV/VIS spectrophotometry, SDS PAGE and isoelectrofocusing. The results of the SDS PAGE suggest that polymerisation of the protein occurs. The shift of the pI of the protein upon modification toward lower values indicates that lysine amino groups are the principal site of the reaction of β-lactoglobulin with the quinines.

【 授权许可】

Unknown