Alpha-pinene and dizocilpine (MK-801) attenuate kindling development and astrocytosis in an experimental mouse model of epilepsy

IBRO Reports

Atsumi Shimada¹  Takeshi Ishihara²  Hiroshi Ueno³  Motoi Okamoto⁴  Naoya Kitamura⁵  Yosuke Matsumoto⁵  Yu Takahashi⁵  Kenta Wani⁵  Shinji Murakami⁵  Shunsuke Suemitsu⁶ 

[1] Corresponding author at: Department of Medical Technology, Kawasaki University of Medical Welfare, 288, Matsushima, Kurashiki, Okayama, 701-0193, Japan.;Department of Medical Technology, Graduate School of Health Sciences, Okayama University, Okayama, 700-8558, Japan;Department of Medical Technology, Kawasaki University of Medical Welfare, Okayama, 701-0193, Japan;Department of Neuropsychiatry, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8558, Japan;Department of Psychiatry, Kawasaki Medical School, Okayama, 701-0192, Japan;Division of Food and Nutrition, Nakamura Gakuen University Junior College, Fukuoka, 814-0198, Japan;

关键词: Epilepsy;    Kindling;    Pentylenetetrazol;    Perineuronal nets;    Wisteria floribunda;   

DOI  :  

来源: DOAJ

【 摘 要 】

Understanding the molecular and cellular mechanisms involved during the onset of epilepsy is crucial for elucidating the overall mechanism of epileptogenesis and therapeutic strategies. Previous studies, using a pentylenetetrazole (PTZ)-induced kindling mouse model, showed that astrocyte activation and an increase in perineuronal nets (PNNs) and extracellular matrix (ECM) molecules occurred within the hippocampus. However, the mechanisms of initiation and suppression of these changes, remain unclear.Herein, we analyzed the attenuation of astrocyte activation caused by dizocilpine (MK-801) administration, as well as the anticonvulsant effect of α-pinene on seizures and production of ECM molecules. Our results showed that MK-801 significantly reduced kindling acquisition, while α-pinene treatment prevented an increase in seizures incidences. Both MK-801 and α-pinene administration attenuated astrocyte activation by PTZ and significantly attenuated the increase in ECM molecules.Our results indicate that astrocyte activation and an increase in ECM may contribute to epileptogenesis and suggest that MK-801 and α-pinene may prevent epileptic seizures by suppressing astrocyte activation and ECM molecule production.

【 授权许可】

Unknown