Systematic Analysis of the Clinical Significance of Hyaluronan-Mediated Motility Receptor in Colorectal Cancer

Frontiers in Molecular Biosciences

Ming-zhi Xie¹  Ke-zhi Li²  Bang-li Hu²  Xin-qiang Liang²  Yi-xin Yin²  Ji-lin Li²  Yan-ping Tang² 

[1] Department of Chemotherapy, Guangxi Medical University Cancer Hospital, Nanning, China;Department of Research, Guangxi Medical University Cancer Hospital, Nanning, China;

关键词: colorectal cancer;    hyaluronan-mediated motility receptor;    tissues;    blood;    cell lines;   

DOI  :  10.3389/fmolb.2021.733271

来源: DOAJ

【 摘 要 】

Background: The role of hyaluronan-mediated motility receptor (HMMR) in colorectal cancer (CRC) remains unclear. The present study aimed to explore the association of HMMR with the development and prognosis of CRC using sequence datasets, clinical tissues, blood samples, and cell lines.Methods: CRC datasets were downloaded from TCGA and GEO databases. Forty CRC tissue samples, 120 CRC blood samples, and 100 healthy controls were collected. Four CRC cell lines (HCT116, HT-29, LoVo, and SW480) and one normal human colon mucosal epithelial cell line (NCM460) were cultured. RT-qPCR was used to determine the expression of HMMR in the tissues and cell lines. ELISA was used to measure HMMR levels in the blood samples.Results: The expression of HMMR was significantly increased in CRC tissues than in corresponding adjacent tissues based on TCGA and GEO datasets, and clinical CRC tissues. No associations were found between the expression of HMMR and the TNM stage or other clinical parameters. The expression of HMMR varied in different CRC cell lines. The blood levels of HMMR tended to be higher in patients with CRC than in healthy controls. TCGA and GEO datasets showed inconsistent results regarding the association of HMMR expression with the survival of patients with CRC.Conclusion: The expression of HMMR is increased in CRC tissues but not in the blood. The expression of HMMR is independent of CRC development and has no prognostic significance in patients with CRC.

【 授权许可】

Unknown