The Distributions and Boundary of Two Distinct, Local Forms of Japanese Pond Frog, Pelophylax porosus brevipodus, Inferred From Sequences of Mitochondrial DNA

Frontiers in Genetics

Ikuo Miura¹  Yukari Nagai²  Toshio Doi³  Takeshi Fujitani⁴  Yoshiaki Yuasa⁵  Kunio Ito⁶  Mitsuaki Ogata⁷  Jun-ichi Naito⁸ 

[1] Amphibian Research Center, Hiroshima University, Higashihiroshima, Japan;Department of Biology, Graduate School of Science, Hiroshima University, Higashihiroshima, Japan;Environmental Assessment and Symbiosis Promotion Division, Kobe Municipal Office, Kobe, Japan;Higashiyama Zoo and Botanical Gardens Information, Nagoya, Japan;Himeji City Aquarium, Himeji, Japan;Kawasaki Senior High School Attached to Kawasaki Medical School, Kurashiki, Japan;Preservation and Research Center, The City of Yokohama, Yokohama, Japan;Society for the Study of Natural History of Nishi-Chugoku Mountains, Hiroshima, Japan;

关键词: Japanese pond frog;    cytochrome b;    D-loop;    SOX3;    two major forms;   

DOI  :  10.3389/fgene.2018.00079

来源: DOAJ

【 摘 要 】

The Nagoya Daruma pond frog Pelophylax porosus brevipodus is distributed in western Japan and is traditionally divided into two local forms: the Okayama form in the west and the Nagoya form in the east. These two forms are genetically differentiated, but have never been defined taxonomically because their distributions are unclear to date. To complete the distributions and identify the boundary of the two forms, we genetically investigated 16 populations including eight populations located within the unexamined area. We found that the distributional boundary is located within a small area of Hyogo Prefecture where haplotypes of mitochondrial cytochrome b (cytb) and D-loop region corresponding to the two forms co-existed. On the other hand, the polymorphic site of the nuclear gene SOX3 revealed introgression over the boundary into Okayama cytb clade. These results suggest that the two forms were geographically isolated from each other in the past, and secondarily contacted and then accepted one-way introgression. As a next step of the research, taxonomic approach is expected to define the two forms.

【 授权许可】

Unknown