Investigating Combined Drought- and Heat Stress Effects in Wheat under Controlled Conditions by Dynamic Image-Based Phenotyping

Agronomy

Bernd Wollenweber¹  Carl-Otto Ottosen²  LamisOsama Anwar Abdelhakim²  Eva Rosenqvist³  Klára Panzarová⁴  Ioannis Spyroglou⁵ 

[1] Department of Agroecology—Crop Health, Aarhus University, Forsøgsvej 1, DK-4200 Slagelse, Denmark;Department of Food Science—Plant, Food & Climate, Aarhus University, Agro Food Park 48, DK-8200 Aarhus N, Denmark;Department of Plant and Environmental Sciences—Crop Sciences, University of Copenhagen, Højbakkegård Allé 13, DK-2630 Tåstrup, Denmark;Photon Systems Instruments, (PSI, spol. sr. o.), 66424 Drásov, Czech Republic;Plant Sciences Core Facility, Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic;

关键词: climate change;    drought;    heat stress;    image-based phenotyping;    RGB imaging;    chlorophyll fluorescence imaging;   

DOI  :  10.3390/agronomy11020364

来源: DOAJ

【 摘 要 】

As drought and heat stress are major challenges for crop productivity under future climate changes, tolerant cultivars are highly in demand. This study investigated the potential of existing Nordic wheat genotypes to resist unfavorable conditions. Four genotypes were selected based on their heat sensitivity (heat-sensitive: LM19, SF1; heat-tolerant: LM62, NS3). At the tillering stage, the plants were subjected to four treatments under controlled conditions: control, drought, heat and combined drought and heat stress. The morpho-physiological performance was quantified during the early and late phase of stress, as well as the recovery phase. We applied an integrative image-based phenotyping approach monitoring plant growth dynamics by structural Red Green Blue (RGB) imaging, photosynthetic performance by chlorophyll fluorescence imaging and transpiration efficiency by thermal infrared imaging. The results demonstrated that the selected genotypes were moderately affected in their photosynthetic efficiency and growth under drought stress, whereas heat and combined stress caused rapid reductions in photosynthesis and growth. Furthermore, drought stress had a major impact on canopy temperature. The NS3 genotype was the most robust genotype, as indicated by its improved response under all stress treatments due to its relatively small biomass. However, the genotypes showed different tolerance to individual and combined stress.

【 授权许可】

Unknown