Fatty acids modulate the expression levels of key proteins for cholesterol absorption in Caco-2 monolayer

Lipids in Health and Disease

Guoxun Chen¹  Jing Li²  Lingjiao Zhu²  Fang Yang²  Ning Qiu²  Meihu Ma² 

[1] Department of Nutrition, University of Tennessee at Knoxville;National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University;

关键词: Fatty acids;    Intestinal cholesterol absorption;    Caco-2 monolayer transport;    NPC1L1;   

DOI  :  10.1186/s12944-018-0675-y

来源: DOAJ

【 摘 要 】

Abstract Background Fatty acids have been shown to modulate intestinal cholesterol absorption in cells and animals, a process that is mediated by several transporter proteins. Of these proteins, Niemann-Pick C1-Like 1 (NPC1L1) is a major contributor to this process. The current study investigates the unknown mechanism by which fatty acids modulate cholesterol absorption. Methods We evaluated the effects of six fatty acids palmitic acid (PAM), oleic acid (OLA), linoleic acid (LNA), arachidonic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on cholesterol uptake and transport in human enterocytes Caco-2 cells, and on the mRNA expression levels of NPC1L1, others proteins (ABCG5, ABCG8, ABCA1, ACAT2, MTP, Caveolin 1, Annexin-2) involved in cholesterol absorption, and SREBP-1 and SREBP-2 that are responsible for lipid metabolism. Results The polyunsaturated fatty acids (PUFAs), especially for EPA and DHA, dose-dependently inhibited cholesterol uptake and transport in Caco-2 monolayer, while saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) had no inhibitory effects. EPA and DHA inhibited cholesterol absorption in Caco-2 monolayer might be caused by down-regulating NPC1L1 mRNA and protein levels, which were associated with inhibition of SREBP-1/− 2 mRNA expression levels. Conclusion Results from this study indicate that functional food containing high PUFAs may have potential therapeutic benefit to reduce cholesterol absorption. Further studies on this topic may provide approaches to control lipid metabolism and to promote health.

【 授权许可】

Unknown