Targeting Reciprocally Connected Brain Regions Through CAV-2 Mediated Interventions

Frontiers in Molecular Neuroscience

Shauna L. Parkes²  Sarah Morceau²  Robin Piquet²  Mathieu Wolff² 

[1] CNRS, INCIA, UMR 5287, Bordeaux, France;Université de Bordeaux, INCIA, UMR 5287, Bordeaux, France;

关键词: neural circuits;    DREADD;    prefrontal cortex;    thalamus;    insular cortex;    basolateral amygdala;   

DOI  :  10.3389/fnmol.2019.00303

来源: DOAJ

【 摘 要 】

An important issue in contemporary neuroscience is to identify functional principles at play within neural circuits. The reciprocity of the connections between two distinct brain areas appears as an intriguing feature of some of these circuits. This organization has been viewed as “re-entry,” a process whereby two or more brain regions concurrently stimulate and are stimulated by each other, thus supporting the synchronization of neural firing required for rapid neural integration. However, until relatively recently, it was not possible to provide a comprehensive functional assessment of such reciprocal pathways. In this Brief Research Report, we highlight the use of a chemogenetic strategy to target projection-defined neurons in reciprocally connected areas through CAV-2 mediated interventions in the rat. Specifically, we targeted the bidirectional pathways between the dorsomedial prefrontal cortex (dmPFC) and the mediodorsal thalamus, as well as those connecting the insular cortex (IC) and the basolateral complex of the amygdala (BLA). These data showcase the usefulness of CAV-2-related strategies to address circuit-level issues. Moreover, we illustrate the inherent limitation of Cre-dependent adeno-associated virues (AAVs) with “leaked” expression of the gene of interest in the absence of Cre and highlight the need for appropriate control conditions.

【 授权许可】

Unknown