Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators

International Journal of Molecular Sciences

Srdjan D. Antic¹  Quyen B. Do²  Thomas Knöpfel²  Chenchen Song² 

[1] Institute for Systems Genomics, Stem Cell Institute, UConn Health, Farmington, CT 06030-3401, USA;Laboratory for Neuronal Circuit Dynamics, Imperial College London, London W12 0NN, UK;

关键词: genetically encoded;    voltage indicator;    intersectional;    transgenic;    inducible expression;    controlled recombination;   

DOI  :  10.3390/ijms18071461

来源: DOAJ

【 摘 要 】

Rapidly progressing development of optogenetic tools, particularly genetically encoded optical indicators, enables monitoring activities of neuronal circuits of identified cell populations in longitudinal in vivo studies. Recently developed advanced transgenic approaches achieve high levels of indicator expression. However, targeting non-sparse cell populations leads to dense expression patterns such that optical signals from neuronal processes cannot be allocated to individual neurons. This issue is particularly pertinent for the use of genetically encoded voltage indicators whose membrane-delimited signals arise largely from the neuropil where dendritic and axonal membranes of many cells intermingle. Here we address this need for sparse but strong expression of genetically encoded optical indicators using a titratable recombination-activated transgene transcription to achieve a Golgi staining-type indicator expression pattern in vivo. Using different transgenic strategies, we also illustrate that co-expression of genetically encoded voltage and calcium indicators can be achieved in vivo for studying neuronal circuit input–output relationships.

【 授权许可】

Unknown