Development of an Improved Method for the Determination of Iodine/β-Cyclodextrin by Means of HPLC-UV: Validation and the Thyroid-Stimulating Activity Revealed by In Vivo Studies

Pharmaceutics

Jamoliddin Razzokov¹  Shokhid Gulyamov¹  Zufar Boboev¹  Avez Sharipov¹  Sunnatullo Fazliev²  Akhmatkhodja Yunuskhodjayev³ 

[1] Department of Inorganic, Physical and Colloidal Chemistry, Tashkent Pharmaceutical Institute, Oybek Street 45, Tashkent 100015, Uzbekistan;Max Planck School Matter to Life, Jahnstraße 29, 69120 Heidelberg, Germany;The Innovation Center of Pharmacy, Tashkent 100015, Uzbekistan;

关键词: iodine;    potassium iodide;    iodine deficiency;    β-cyclodextrin;    iodine/β-CD;    thyroid-stimulating activity;   

DOI  :  10.3390/pharmaceutics13070955

来源: DOAJ

【 摘 要 】

Iodine, being an intrinsic part of thyroid hormones, is a vital microelement required for normal growth and development, particularly in children. Inadequate daily intake of iodine causes iodine deficiency, which is responsible for several health disorders, such as cretinism and goiters. Therefore, the development of new drugs and/or food supplements for iodine deficiency is crucial. We synthesized an iodine/β-cyclodextrin complex based on a host–guest model, and in this paper, we outline the development of a new quantitative analysis method. We suggest a robust and reliable high-performance liquid chromatography method to determine the total amount of iodine species in the complex. Moreover, we performed validation of our method. The results of validation presented here show the reliability, accuracy and high precision of the method. Furthermore, for the first time, we show results of in vivo studies for the thyroid-stimulating activity of the iodine/β-cyclodextrin complex. Our findings indicate that the thyroid-stimulating activity of iodine/β-cyclodextrin is comparable to that of potassium iodide, which is the main active pharmaceutical substance of conventional drugs for iodine deficiency.

【 授权许可】

Unknown