Isolation and Identification of Potent Antidiabetic Compounds from Antrodia cinnamomea—An Edible Taiwanese Mushroom

Molecules

HungTse Huang¹  San-Lang Wang²  Yao-Haur Kuo³  VanBon Nguyen⁴ 

[1] Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan;Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan;Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei 11221, Taiwan;Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam;

关键词: Antrodia cinnamomea;    antidiabetic;    edible mushroom;    α-glucosidase inhibitor;    antcin K;    dehydrosulphurenic acid;    dehydroeburicoic acid;    eburicoic acid;   

DOI  :  10.3390/molecules23112864

来源: DOAJ

【 摘 要 】

Antrodia cinnamomea (AC), an edible Taiwanese mushroom, has been recognized as a valuable natural resource with vast biological and medicinal benefits. Recently, the hypoglycemic and anti-diabetic effects of AC were mentioned in several studies. However, no studies have investigated α-glucosidase inhibitors from AC fruiting bodies (ACFB) as they relate to type 2 diabetes (T2D) treatment. The purpose of this study was to gain evidence of potent α-glucosidase inhibitory effects, as well as isolate, identify and characterize the active compounds of ACFB. The MeOH extract of ACFB demonstrated potent α-glucosidase inhibitory activity, and possessed high pH stability (pH 2⁻11) and thermostable properties at 40⁻50 °C. Further purification led to the isolation of eight constituents from ACFB, identified as: 25S-antcin K (1), 25R-antcin K (2), dehydrosulphurenic acid (3), 25S-antcin I (4), 25S-antcin B (5), 25R-antcin B (6), dehydroeburicoic acid (7) and eburicoic acid (8). Notably, the ACFB extract and its identified compounds, except 1, 4, and 6 demonstrated a greater effect (EC₅₀ = 0.025⁻0.21 mg/mL) than acarbose (EC₅₀ = 0.278 mg/mL). As such, these active compounds were determined to be new potent mushroom α-glucosidase inhibitors. These active compounds were also identified on the HPLC fingerprints of ACFB.

【 授权许可】

Unknown