Morphological and Molecular Investigation of Anaplasma Infection in Dromedary Camel (Camelus dromedarius) in Bushehr Province, Iran

Iranian Journal of Veterinary Medicine

Feisal Zarghami¹  Zahra Moradi²  Parviz Shayan³  Elahe Ebrahimzadeh⁴ 

[1] Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran- Ira;Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran- Iran;Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran;

关键词: anaplasma;    candidatus anaplasma camelii;    dromedary camel;    molecular study;    nested-pcr;   

DOI  :  10.22059/ijvm.2020.306095.1005109

来源: DOAJ

【 摘 要 】

BACKGROUND: Anaplasmosis is caused by an obligate intracellular, gram-negative microorganism, which be-longs to the family Anaplasmatacea and can be transmitted by ticks and other arthropods.OBJECTIVES: The present study aimed to investigate the status of Anaplasma spp. infection by microscopy and molecular methods in dromedary camels in Bushehr province, Iran.METHODS: A total of 139 blood samples were collected from dromedary camels in Bushehr province. Giemsa staining and nested-polymerase chain reaction (PCR) were conducted to detect Anaplasma infection in the drome-dary camels.RESULTS: We found that 27 (19.4%) out of the total 139 blood samples were suspected for the presence of Ana-plasma spp. by morphological study. The PCR and nested-PCR sequencing results showed 111 (80%) and 134 (96%) samples positive for Anaplasma spp. and BLAST search in NCBI GenBank presented 100% identity with Candidatus Anaplasma camelii.CONCLUSIONS: The molecular results presented the high frequency of Candidatus Anaplasma camelii in camels, in Bushehr city.

【 授权许可】

Unknown