Circular RNA CircPPP1CB Suppresses Tumorigenesis by Interacting With the MiR-1307-3p/SMG1 Axis in Human Bladder Cancer

Frontiers in Cell and Developmental Biology

Mengjing Fan¹  Guoqing Ding²  Yanlan Yu²  Xiaodong Jin³  Xianwu Chen³  Yueshu Cai³  Hongshen Wu³  Feifan Wang³  Jiayong Xiang³  Shihan Huang³  Ning He³  Zhenghui Hu³  Yan Zhang³  Xuejian Zhou³ 

[1] Department of Pathology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China;Department of Urology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China;Department of Urology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China;

关键词: circPPP1CB;    bladder cancer;    miR-1307-3p;    SMG1;    tumorigenesis;   

DOI  :  10.3389/fcell.2021.704683

来源: DOAJ

【 摘 要 】

Circular RNA (circRNA) is a newly discovered endogenous non-coding RNA (ncRNA), which is characterized with a closed circular structure. A growing body of evidence has verified the vital roles of circRNAs in human cancer. In this research, we selected circPPP1CB as a study object by circRNA sequencing and quantitative real-time PCR (qRT-PCR) validation in human bladder cancer (BC). CircPPP1CB is downregulated in BC and is negatively correlated with clinical stages and histological grades. Functionally, circPPP1CB modulated cell growth, metastasis, and epithelial-to-mesenchymal transition (EMT) process in vitro and in vivo. Mechanically, we performed various experiments to verify the circPPP1CB/miR-1307-3p/SMG1 regulatory axis. Taken together, our results demonstrated that circPPP1CB participates in tumor growth, metastasis, and EMT process by interacting with the miR-1307-3p/SMG1 axis, and that circPPP1CB might be a novel therapeutic target and diagnostic biomarker in human BC.

【 授权许可】

Unknown