Cytotoxicity Study of UV-Laser-Irradiated PLLA Surfaces Subjected to Bio-Ceramisation: A New Way towards Implant Surface Modification

International Journal of Molecular Sciences

Patrycja Szymczyk-Ziółkowska¹  Viktoria Hoppe¹  Włodzimierz Tylus²  Marta Tikhomirov³  Błażej Poźniak³  Bartłomiej Kryszak⁴  Konrad Szustakiewicz⁴  Michał Grzymajło⁴  Agnieszka Gadomska-Gajadhur⁵  Paulina Dzienny⁶  Arkadiusz J. Antończak⁶ 

[1] Centre for Advanced Manufacturing Technologies, Faculty of Mechanical Engineering, Wrocław University of Science and Technology (WUST), Łukasiewicza 5, 50-370 Wrocław, Poland;Department of Advanced Material Technologies, Faculty of Chemistry, Wrocław University of Science and Technology, 50-370 Wrocław, Poland;Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, ul. Norwida 25, 50-375 Wrocław, Poland;Department of Polymer Engineering and Technology, Faculty of Chemistry, Wrocław University of Science and Technology (WUST), Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland;Faculty of Chemistry, Warsaw University of Technology, ul. Noakowskiego 3, 00-664 Warsaw, Poland;Laser and Fiber Electronics Group, Faculty of Electrical Engineering, Wrocław University of Science and Technology, 50-370 Wrocław, Poland;

关键词: polymers;    poly(L-Lactide);    laser modification;    extrusion casting;    selective biomineralization;    fibroblasts;   

DOI  :  10.3390/ijms22168436

来源: DOAJ

【 摘 要 】

In this research we subjected samples of poly(L-lactide) (PLLA) extruded film to ultraviolet (193 nm ArF excimer laser) radiation below the ablation threshold. The modified film was immersed in Simulated Body Fluid (SBF) at 37 °C for 1 day or 7 days to obtain a layer of apatite ceramic (CaP) coating on the modified PLLA surface. The samples were characterized by means of optical profilometry, which indicated an increase in average roughness (Ra) from 25 nm for the unmodified PLLA to over 580 nm for irradiated PLLA incubated in SBF for 1 day. At the same time, the water contact angle decreased from 78° for neat PLLA to 35° for irradiated PLLA incubated in SBF, which suggests its higher hydrophilicity. The obtained materials were investigated by means of cell response fibroblasts (3T3) and macrophage-like cells (RAW 264.7). Properties of the obtained composites were compared to the unmodified PLLA film as well as to the UV-laser irradiated PLLA. The activation of the PLLA surface by laser irradiation led to a distinct increase in cytotoxicity, while the treatment with SBF and the deposition of apatite ceramic had only a limited preventive effect on this harmful impact and depended on the cell type. Fibroblasts were found to have good tolerance for the irradiated and ceramic-covered PLLA, but macrophages seem to interact with the substrate leading to the release of cytotoxic products.

【 授权许可】

Unknown