Route of Zika virus infection in Aedes aegypti by transmission electron microscopy

BMC Microbiology

Tiago Souza Salles¹  Monica Ferreira Moreira¹  Thayane da Encarnação Sá-Guimarães¹  Lucio Ayres Caldas²  Carlucio Rocha dos Santos³  Wanderley de Souza⁴ 

[1] Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro;Instituto de Biofísica Carlos Chagas Filho, Laboratório de Ultraestrutura Celular Hertha Meyer, Universidade Federal do Rio de Janeiro;Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz;Universidade Federal do Rio de Janeiro, Centro Nacional de Biologia Estrutural e Bioimagem (CENABIO);

关键词: Zika virus;    Aedes aegypti;    Transmission Electron Microscopy;    Eggs;    Ovary;    Vertical transmission;   

DOI  :  10.1186/s12866-021-02366-0

来源: DOAJ

【 摘 要 】

Abstract Background Zika fever has been a global health security threat, especially in the tropical and subtropical regions where most of the cases occur. The disease is caused by Zika virus (ZIKV), which belongs to the family Flaviviridae, genus Flavivirus. The virus is transmitted by Aedes mosquitoes, mostly by Aedes aegypti, during its blood meal. In this study we present a descriptive analysis, by transmission electron microscopy (TEM), of ZIKV infection in A. aegypti elected tissues at the 3rd day of infection. ZIKV vertical transmission experiments by oral infection were conducted to explore an offspring of natural infection. Results Gut and ovary tissues harbored a higher number of viral particles. The ZIKV genome was also detected, by RT-qPCR technique, in the organism of orally infected female mosquitoes and in their eggs laid. Conclusions The data obtained suggest that the ovary is an organ susceptible to be infected with ZIKV and that virus can be transmitted from mother to a fraction of the progeny.

【 授权许可】

Unknown