Tomato Chlorosis Virus Infection Facilitates Bemisia tabaci MED Reproduction by Elevating Vitellogenin Expression

Insects

Xuguo Zhou¹  Jizhe Shi¹  Yong Fang²  Yang Gao³  Zhuo Zhang³  Limin Zheng³  Xinqiu Tan³  Zhanhong Zhang⁴  Xiaobin Shi⁵  Yong Liu⁵  Deyong Zhang⁵  Liping Huang⁵  Qiuyi Pan⁶ 

[1] Department of Entomology, University of Kentucky, Lexington, KY 40546, USA;Institute of Biotechnology, Hunan Academy of Agricultural Sciences, Changsha 410125, China;Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China;Institute of Vegetable, Hunan Academy of Agricultural Sciences, Changsha 410125, China;Longping Branch Graduate School, Hunan University, Changsha 410125, China;Plant Protection and Plant Inspection Station, Changde 415000, China;

关键词: Bemisia tabaci;    fecundity;    ovarian development;    gene expression;    RNA interference;    vitellogenin;   

DOI  :  10.3390/insects12020101

来源: DOAJ

【 摘 要 】

Transmission of plant pathogenic viruses mostly relies on insect vectors. Plant virus could enhance its transmission by modulating the vector. Previously, we showed that feeding on virus infected plants can promote the reproduction of the sweet potato whitefly, Bemisia tabaci MED (Q biotype). In this study, using a whitefly-Tomato chlorosis virus (ToCV)-tomato system, we investigated how ToCV modulates B. tabaci MED reproduction to facilitate its spread. Here, we hypothesized that ToCV-infected tomato plants would increase B. tabaci MED fecundity via elevated vitellogenin (Vg) gene expression. As a result, fecundity and the relative expression of B. tabaci MED Vg was measured on ToCV-infected and uninfected tomato plants on days 4, 8, 12, 16, 20 and 24. The role of Vg on B. tabaci MED reproduction was examined in the presence and absence of ToCV using dietary RNAi. ToCV infection significantly increased B. tabaci MED fecundity on days 12, 16 and 20, and elevated Vg expression on days 8, 12 and 16. Both ovarian development and fecundity of B. tabaci MED were suppressed when Vg was silenced with or without ToCV infection. These combined results suggest that ToCV infection increases B. tabaci MED fecundity via elevated Vg expression.

【 授权许可】

Unknown