Receptor-Mediated Delivery of Astaxanthin-Loaded Nanoparticles to Neurons: An Enhanced Potential for Subarachnoid Hemorrhage Treatment

Frontiers in Neuroscience

Sheng Cui¹  Xiang-long Tang¹  Zong-qi You²  Xin Zhang³  Li-li Wen³  Bin Yuan³  Qi Wu⁴  Xiang-sheng Zhang⁴  Xiao-ming Zhou⁵  Wei-dong Xu⁶ 

[1] College of Material Sciences and Engineering, Nanjing Tech University, Nanjing, China;Department of Neurosurgery, School of Medicine, Jinling Hospital, Jiangsu University, Zhenjiang, China;Department of Neurosurgery, School of Medicine, Jinling Hospital, Nanjing Medical University, Nanjing, China;Department of Neurosurgery, School of Medicine, Jinling Hospital, Nanjing University, Nanjing, China;Department of Neurosurgery, School of Medicine, Jinling Hospital, Second Military Medical University, Shanghai, China;Department of Neurosurgery, School of Medicine, Jinling Hospital, Southern Medical University (Guangzhou), Nanjing, China;

关键词: astaxanthin;    transferrin;    nanoparticle;    targeted delivery;    neuroprotection;   

DOI  :  10.3389/fnins.2019.00989

来源: DOAJ

【 摘 要 】

Astaxanthin (ATX) is a carotenoid that exerts strong anti-oxidant and anti-inflammatory property deriving from its highly unsaturated molecular structures. However, the low stability and solubility of ATX results in poor bioavailability, which markedly hampers its application as therapeutic agent in clinic advancement. This study investigated a promising way of transferrin conjugated to poly (ethylene glycol) (PEG)-encapsulated ATX nanoparticles (ATX-NPs) on targeted delivery and evaluated the possible mechanism underlying neuroprotection capability. As a result, the ATX integrated into nanocarrier presented both well water-dispersible and biocompatible, primely conquering its limitations. More than that, the transferrin-containing ATX-NPs exhibited enhanced cellular uptake efficiency than that of ATX-NPs without transferrin conjugated in primary cortical neurons. Additionally, compared to free ATX, transferrin-containing ATX-NPs with lower ATX concentration showed powerful neuroprotective effects on OxyHb-induced neuronal damage. Taken together, the improved bioavailability and enhanced neuroprotective effects enabled ATX-NPs as favorable candidates for targeted delivery and absorption of ATX. We believe that these in vitro findings will provide insights for advancement of subarachnoid hemorrhage therapy.

【 授权许可】

Unknown