Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial

Cells

Lucía Murria¹  Ana Ortiz-Vallecillo¹  Alberto Pacheco²  Manuel Fernández-Sánchez³  Julia Ramos³  Francisco Carranza³  Cristina González-Ravina³  Esther Santamaría-López³ 

[1] IVI Foundation, Instituto de Investigación Sanitaria La Fe (IIS La Fe), 46026 Valencia, Spain;IVI-RMA Madrid, Avenida del Talgo 68, 28023 Madrid, Spain;IVI-RMA Seville, Avda. Américo Vespucio 19, 41092 Seville, Spain;

关键词: MACS;    sperm;    sperm selection;    IUI;    sperm DNA fragmentation;   

DOI  :  10.3390/cells11111794

来源: DOAJ

【 摘 要 】

Clinical outcome in assisted reproduction techniques (ARTs) is mainly influenced by the quality of gametes used. It is known that a high percentage of sperm DNA fragmentation (DNAf) decreases the success of ART clinical results. Therefore, techniques such as magnetic-activated cell sorting (MACS) help to improve results in cases of patients with a high percentage of DNAf. Cryopreservation of sperm in donor intrauterine insemination (D-IUI) treatments increases sperm DNAf, so patients using these sperm samples can benefit from using this technique. This prospective randomized national multicenter study analyzed clinical outcomes of 181 D-IUI treatments. MACS was performed after density gradient centrifugation (DGC) in 90 thawed semen donor samples (MACSG), whereas only DGC was performed in 91 thawed semen donor samples (CG). To our knowledge, this is the first study analyzing the effect of MACS on D-IUI cycles. Our results show no significant differences in gestation, live birth, or miscarriage rates between the two groups. We believe that further studies with a larger sample size are needed to evaluate the application of MACS in combination with standard IUI donor sperm preparations in fertility clinics.

【 授权许可】

Unknown