RNA-seq profiling reveals differentially expressed genes as potential markers for vital reaction in skin contusion: a pilot study

Forensic Sciences Research

Rui Zhao¹  Yanliang Sheng²  Dong Zhao³  Hongyan Huang⁴  Huijun Wang⁴  Jietao He⁴  Jingtao Xu⁴  Qi Wang⁴  Ye Xue⁴  Huanqin Xiao⁵ 

[1] China Medical University School of Forensic Medicine;Jiamusi University;Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education;Southern Medical University;Sun Yat-Sen University;

关键词: Forensic pathology;    skin contusion;    vital reaction;    RNA-seq;    differentially expressed genes;   

DOI  :  10.1080/20961790.2017.1349639

来源: DOAJ

【 摘 要 】

Detection of the vitality of wounds is essential in forensic practice. The present study used Illumina RNA-seq technology to determine gene expression profiles in contused mouse skin. In obtained high quality sequencing reads, the reads were mapped onto a reference transcriptome (Mus_musculus.GRCm38.83). The results revealed that there were 659 up-regulated and 996 down-regulated differentially expressed genes (DEGs) in contused mouse skin. The DEGs were further analyzed using the Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes databases. Genes from different functional categories and signalling pathways were enriched, including the immune system process, immune response, defense response, cytokine–cytokine receptor interaction, complement and coagulation cascades and chemokine signalling pathway. Expression patterns of 11 DEGs were verified by RT-qPCR in mice skins. In addition, alterations of five DEGs were also analyzed in postmortem human wound samples. The results were in concordance with the results of RNA-seq. These findings suggest that RNA-seq is a powerful tool to reveal DEGs as potential markers for vital reaction in terms of forensic practices.

【 授权许可】

Unknown