Deciphering the microbiota of Tuwa hot spring, India using shotgun metagenomic sequencing approach

Genomics Data

Prakash Koringa¹  C.G. Joshi¹  Amitsinh Mangrola²  Pravin Dudhagara³  Mansi Parmar⁴  Rajesh Patel⁴ 

[1] Department of Animal Biotechnology, Anand Agricultural University, Anand, Gujarat, India;Department of Biochemistry, Shri Alpesh N. Patel Post Graduate Institute, Anand, India;Department of Biotechnology, Veer Narmad South Gujarat University, Surat, India;Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, India;

关键词: Hot spring;    Metagenome;    Shotgun sequencing;    Community analysis;    Functional analysis;   

DOI  :  10.1016/j.gdata.2015.04.014

来源: DOAJ

【 摘 要 】

Here, we report metagenome from the Tuwa hot spring, India using shotgun sequencing approach. Metagenome consisted of 541,379 sequences with 98.7 Mbps size with 46% G + C content. Metagenomic sequence reads were deposited into the EMBL database under accession number ERP009321. Community analysis presented 99.1% sequences belong to bacteria, 0.3% of eukaryotic origin, 0.2% virus derived and 0.05% from archea. Unclassified and unidentified sequences were 0.4% and 0.07% respectively. A total of 22 bacterial phyla include 90 families and 201 species were observed in the hot spring metagenome. Firmicutes (97.0%), Proteobacteria (1.3%) and Actinobacteria (0.4%) were reported as dominant bacterial phyla. In functional analysis using Cluster of Orthologous Group (COG), 21.5% drops in the poorly characterized group. Using subsystem based annotation, 4.0% genes were assigned for stress responses and 3% genes were fit into the metabolism of aromatic compounds. The hot spring metagenome is very rich with novel sequences affiliated to unclassified and unidentified lineages, suggesting the potential source for novel microbial species and their products.

【 授权许可】

Unknown