Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-phiIPLA7 as an anti-biofilm agent in staphylococcal species

Frontiers in Microbiology

Pilar eGarcía¹  Ana eRodríguez¹  Diana eGutiérrez¹  Beatriz eMartínez¹  Lorena eRodríguez-Rubio²  Rob eLavigne²  Yves eBriers² 

[1] Consejo Superior de Investigaciones Científicas, CSIC;Laboratory of Gene Technology, KU Leuven;

关键词: Biofilm;    S. aureus;    S. epidermidis;    Biofilm matrix;    Exopolysaccharide depolymerase;   

DOI  :  10.3389/fmicb.2015.01315

来源: DOAJ

【 摘 要 】

Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The exopolysaccharide depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in E. coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (>90%) of biofilm-attached cells was obtained with 0.15 µM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded exopolysaccharide depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials.

【 授权许可】

Unknown