Rhizosphere Bacteria From Panax notoginseng Against Meloidogyne hapla by Rapid Colonization and Mediated Resistance

Frontiers in Microbiology

Shusheng Zhu¹  Youyong Zhu¹  Yang Wang¹  Jingjing Wang¹  Wentao Wu¹  Liwei Guo¹  Zhuhua Wang¹  Xiahong He² 

[1] Key Laboratory of Agricultural Biodiversity and Pest Control, College of Plant Protection, Yunnan Agricultural University, Kunming, China;School of Landscape and Horticulture, Southwest Forestry University, Kunming, China;

关键词: rhizosphere bacteria;    biological control;    Meloidogyne hapla;    rapid colonization;    resistance induction;   

DOI  :  10.3389/fmicb.2022.877082

来源: DOAJ

【 摘 要 】

Root-knot nematodes (RKNs) are soil-borne pathogens that severely affect Panax notoginseng growth and productivity. Thus, there is an urgent need for biological control agents or green nematicides to control root-knot nematodes. Rhizosphere bacteria can effectively control RKNs through different mechanisms. In this study, the three rhizosphere Bacillus strains, isolated from the root of P. notoginseng, were evaluated for the nematicidal activity and biological control efficacy against root-knot nematodes. In addition, we also evaluated the colonization ability of the two bacterial strains with significant biocontrol effect and dynamic regulation of genes related to systemic resistance in P. notoginseng. The rhizosphere Bacillus velezensis GJ-7 and Bacillus cereus NS-2 showed high nematicidal activity against Meloidogyne hapla in vitro and significantly reduced the number of root galls in three different control experiments. The results of colonization experiments showed that the strains GJ-7 and NS-2 colonized P. notoginseng root rapidly and stably. Additionally, the colonization of the strains NS-2 and GJ-7 activated the defense-responsive genes in P. notoginseng. These results indicated that the B. cereus strain NS-2 and B. velezensis strain GJ-7 have the potential for successful ecological niche occupation and enhance plant resistance and therefore could be considered as potential biocontrol agents against root-knot nematodes.

【 授权许可】

Unknown