Efficacy and Cytotoxicity of Binary Mixtures as Root Canal Filling Solvents

Materials

Irene Pina-Vaz¹  AnaCristina Braga²  Inês Ferreira³  MariaAscensão Lopes⁴  Pedro Gomes⁵  Liliana Grenho⁵  MariaHelena Fernandes⁵ 

[1] CINTESIS, Faculdade de Medicina Dentária, Universidade do Porto, 4200-393 Porto, Portugal;Departamento de Produção e Sistemas da Escola de Engenharia, Centro ALGORITMI, Universidade do Minho, 4710-057 Braga, Portugal;Faculdade de Medicina, Universidade do Porto, 4200-319 Porto, Portugal;LAQV/REQUIMTE, U.Porto, 4160-007 Porto, Portugal;Laboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária, Universidade do Porto, 4200-393 Porto, Portugal.;

关键词: endodontic material;    epoxy resin-based root canal sealer;    gutta-percha;    retreatment;    root canal filling materials;    solvents;   

DOI  :  10.3390/ma13143237

来源: DOAJ

【 摘 要 】

Objectives: This study reports the efficacy of two solvent mixtures on the dissolution of gutta-percha and AH Plus sealer, together with the cytotoxicity. Methods: Methyl ethyl ketone (MEK), orange oil, tetrachloroethylene, MEK/tetrachloroethylene (1:1), MEK/orange oil (1:1), and chloroform (control) were tested. Twelve groups (n = 15) of standardized stainless-steel molds filled with softened gutta-percha cones and twelve (n = 15) filled with AH Plus were immersed in the corresponding mixture or individual solvent, in an ultrasonic bath, for either 2 or 5 min. The effect of the solvents was assessed qualitatively by a topographical analysis (scanning electron microscopy) and chemical analysis (Fourier transform infrared spectroscopy), and quantitatively by a weight loss and viscoelastic property (dynamic mechanical analysis) evaluation. The cytotoxicity was assessed on MG63 human osteoblastic cells. Results: The mixtures did not show the formation of new compounds. Both presented significantly higher efficacies compared to their individual solvents, suggesting a synergistic effect. Their dissolution efficacy was similar to that of chloroform, showing high cytocompatibility. Conclusions: The proposed strategy, incorporating ultrasound agitation and profiting from the synergy of adequate solvents, might enhance root canal cleanliness allowing a single-step procedure to dissolve gutta-percha and the sealer remnants, while assuring cytocompatibility with the periapical tissues.

【 授权许可】

Unknown