PVA-Based Electrospun Biomembranes with Hydrolyzed Collagen and Ethanolic Extract of Hypericum perforatum for Potential Use as Wound Dressing: Fabrication and Characterization

Polymers

Eduardo Morales-Sánchez¹  Monserrat Escamilla-García²  Alitzel Belém García-Hernández³  Georgina Calderón-Domínguez³  Miguel A. Vega-Cuellar³  Blanca M. Berdeja-Martínez³  Reynold R. Farrera-Rebollo³  Ma. Paz Salgado-Cruz³  Minerva Rentería-Ortega⁴ 

[1] Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, Unidad Querétaro, Instituto Politécnico Nacional, Qro., Santiago de Querétaro 76090, Mexico;Departamento de Investigación en Alimentos y Estudios de Postgrado, Universidad Autónoma de Querétaro, Qro., Santiago de Querétaro 76010, Mexico;Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, CDMX, Mexico City 07738, Mexico;Tecnológico Nacional de México/TES de San Felipe del Progreso, Edo. Méx., San Felipe del Progreso 50640, Mexico;

关键词: Hypericum perforatum;    hydrolyzed collagen;    polyvinyl alcohol;    electrospinning;    wound dressing;   

DOI  :  10.3390/polym14101981

来源: DOAJ

【 摘 要 】

Biological, physicochemical, structural, and thermal properties of PVA-based electrospun wound dressings added with hydrolyzed collagen (HC) and different concentrations of Hypericum perforatum ethanolic extract (EEHP) were studied. Membrane characterization was carried out by X-ray diffraction, Fourier infrared spectroscopy, differential scanning calorimetry, barrier properties, scanning electron microscopy, image analysis (diameter and pore size), as well as antimicrobial and anti-inflammatory activities. Results showed that the PVA/HC/EEHP materials, fabricated under controlled conditions of temperature and humidity, generated fiber membranes with diameters between 140–390 nm, adequate porosity and pore size for cell growth (67–90% and 4–16 µm, respectively), and good barrier properties (0.005–0.032 g·m⁻² s⁻¹) to be used in the treatment of conditions on the skin, and was even better than some commercial products. Finally, they showed to have anti-inflammatory (>80%), and antimicrobial activity against S. aureus and S. epiderm. Furthermore, higher crystalline structure was observed according to the EEHP concentration. In addition, this is the first report in which PVA/HC/EEHP membranes are successfully fabricated and characterized.

【 授权许可】

Unknown