Acute Ethanol inhibition of γ Oscillations is mediated by Akt and GSK-3β

Frontiers in Cellular Neuroscience

Martin Vreugdenhil¹  JingXi Zhao²  ZhiHua Liu²  RuiLing Zhang²  Chengbiao Lu³  YaLi Wang³  XiaoFang Wang³  FangLi Guo³  JianGang Wang³ 

[1] Birmingham City University;Psychiatric Hospital of Henan Province;Xinxiang Medical University;

关键词: Akt;    Dopamine receptor;    NMDA receptor;    PKA;    GSK3β;    γ oscillation;   

DOI  :  10.3389/fncel.2016.00189

来源: DOAJ

【 摘 要 】

Hippocampal network oscillations at gamma band frequency (γ, 30-80 Hz) are closely associated with higher brain functions such as learning and memory. Acute ethanol exposure at intoxicating concentrations (≥50mM) impairs cognitive function. This study aimed to determine the effects and the mechanisms of acute ethanol exposure on γ oscillations in an in vitro model. Ethanol (25-100 mM) suppressed kainate-induced γ oscillations in CA3 area of the rat hippocampal slices, in a concentration-dependent, reversible manner. The ethanol-induced suppression was reduced by the D1R antagonist SCH23390 or the PKA inhibitor H89, was prevented by the Akt inhibitor Triciribine or the GSk3β inhibitor SB415286, was enhanced by the NMDA receptor antagonist D-AP5, but was not affected by the MAPK inhibitor U0126 or PI3K inhibitor wortmanin. Our results indicate the intracellular kinases Akt and GSk3β play a critical role in the ethanol-induced suppression of γ oscillations and reveal new cellular pathways involved in the ethanol-induced cognitive impairment.

【 授权许可】

Unknown