Optimization of protein production by Geotrichum candidum MIUG 2.15 by cultivation on paper residues, using response surface methodology

BioResources

关键词: Geotrichum candidum;    Protein production;    Solid-state fermentation;    Paper residues;    Response surface methodology (RSM);   

DOI  :  

来源: DOAJ

【 摘 要 】

Response surface methodology (RSM) based on the 23 factorial central composite design (CCD) was used to optimize the biotechnological conditions for growth and protein production by a selected fungal strain Geotrichum candidum MIUG 2.15, by solid-state cultivation on a semisolid medium based on a mixture of paper residues, i.e. office paper, newspaper, and cardboard, mixed in a ratio of 1:1:1(w/w), supplemented with cheese whey waste and complex manure. Three independent variables, the solid:liquid ratio, the concentration of complex manure, and cultivation time, were evaluated to determine their correlative effect on biomass production and protein biosynthesis. The optimal conditions for obtaining a maximum protein yield of 9.53% w/w dry mass were the following: the complex manure concentration of 0.5%, the solid:liquid ratio of 1:5, and the growth time of 10 days.

【 授权许可】

Unknown