Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro

Journal of Biological Researches

Aryani Leksonowati¹  Tatin Suherlina¹  Witjaksono Witjaksono¹ 

[1] Pusat Penelitian Biologi LIPI, Cibinong Science Center, Jl Raya Bogor-Jakarta Km 46, Cibinong, Bogor 16911 Telp/Fax: [phone];

关键词: in vitro culture;    leaf position;    organogenesis;    Solenostemon rotundifolius (Poir) JK Morton;    Culture age;    In vitro culture;    Leaf position;    Organogenesis;   

DOI  :  10.23869/bphjbr.17.1.201119

来源: DOAJ

【 摘 要 】

Plant regeneration via somatic embryogenesis or organogenesis is a key for manipulation of somatic cells in vitro. Organogenesisfrom leaf inoculum, petiole and stem segments of in vitro black potato (Solenostemon rotundifolius (Poir) JK Morton) is influencedby the balance of the concentration of BA and NAA in the culture medium. However, organogenesis could also be affected by factorsinherent to the inoculums. This paper reports the influence of leaf position on the in vitro shoots and culture age on the formation andproduction of shoot adventives from leaf inoculum. Leaves excised from shoots of 3-8 week-old culture and leaf position 1-5 from apicalto basal was used as inocula. The highest percentage (83.3%) of adventitious bud develops at cultures age of 5 weeks with the averagenumber of shoots 6.1. The highest percentage of bud development occurs at leaf position number 2-4, with the percentage of buds of83.3, 76.2 and 76.2, respectively with the average number of shoots of 3.19, 2.10 dan 2.38 respectively. Organogenesis that producesthe highest shoot production of 18.6 per Petri dish was obtained from leaves derived from the 2ndleaf from apical of 5 week-old cultureinoculated on MS medium enriched with 5 mg/l BA and 0.1 mg/l NAA.

【 授权许可】

Unknown